eIF2α of Litopenaeus vannamei involved in shrimp immune response to WSSV infection
Published:2014-12-11 14:04:55    Text Size:【BIG】【MEDIUM】【SMALL
Summary:The alpha subunit of Eukaryotic Initiation Factor 2 (eIF2a) is a key translation regulator that plays an important role in cellular stress responses, which including virus infection. To investigate whether WSSV infection can activate the PERK-eIF2a pathway, the eIF2a in shrimp Litopenaeus vannamei, designed as LveIF2a, was analyzed. The LveIF2a, a 332-amino acid polypeptide, shares a high degree of similarity with eIF2a from other species, having two eIF2a protein signatures at the 13-88 aa and 192-243 aa. The WSSV challenge experiment showed that the protein level of the total LveIF2a was decreased after infection, while the phosphorylation of LveIF2a has no significant change, which indicated that the phosphorylation ratio of LveIF2a was increased after infection. Furthermore, inhibitor treatment led to a significant decrease of WSSV loads. Moreover, the Binding immunoglobulin protein (BiP), an endoplasmic reticulum (ER) stress sensor, and PERK were also investigated during virus infection and it was shown that they were both up-regulated. Taken together, these results suggested that WSSV infection can induce ER stress and activated the unfolded protein response (UPR), and the PERK-eIF2a pathway is important for innate immune during WSSV infection in shrimp.
Xu J, Ruan L, Shi H. Fish Shellfish Immunol, 2014, 40(2): 609-615.